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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
on mouse tissue are of IgG1 isotype. Detailed explanation of this method can be found in: Cattoretti G, Qing Fei. Application of the Antigen Retrieval Technique in experimental Pathology: from human to mouse
The production of monoclonal antibody by hybridoma
cell culture plates. Reagents: 1-3 x 10 8-9 immune spleen cells 1-6 x10 7-8 myeloma cells in log phase of growth Complete Media No Sera (CMNS) for washing of the myeloma and spleen cells. Hybridoma medium CM - HAT
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