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for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
The production of monoclonal antibody by hybridoma
and grow up for ascites/ large flask production and further freeze down. Troubleshooting I strongly reccommend to use Southern Biotech Goat anti-Mouse Ig (H+L
volume to bring the cells to the following concentrations: Type Cell Concentration mouse or rat 1.5 x 106 cells per ml hamster 0.5 - 1.0 x 106 cells per ml
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