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- 2025年07月14日
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文献和实验Chromatin Immunoprecipitation (ChIPs) Cloning Protocol (Farnham Lab)
). Day 2 14. If you are not using rabbit polyclonal antibodies (ie. monoclonal antibodies, ect), add 1 ug of an appropriate secondary antibody (rabbit) and incubate for an additional 1 hour. 15. Add
Large-scale immunofluorescence
containing 2% n-propyl gallate and 0.25 ug/ml Hoechst Primary antibodies rabbit anti-beta-gal antibody (Cappell Laboratories #0631-0002, at 1:200 dilution) rat monoclonal anti-yeast tubulin (YOL1/34; Sera-lab, at 1:100 dilution
2 n-propyl gallate and 0.25 ug/ml Hoechst Primary antibodies rabbit anti-beta-gal antibody (Cappell Laboratories #0631-0002, at 1:200 dilution) rat monoclonal anti-yeast tubulin (YOL1/34; Sera-lab, at 1:100 dilution
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