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0.1 mg
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文献和实验A c-Kit (2B8) FITC-IgG2b, 01904D FITC-IgG2b Isotype control, 11184C Fc RII block (clone 2.4G2), 01241D Media: Heat inactivated FBS (56°C x 30 min) PBS + 2% heat-inactivated
Intracellular Immunofluorescent Staining for Flow Cytometry
-7178 ). Isotype control on the left and anti-IL-17 on the right. Cells in the lymphocytes gate were used for analysis. Intracellular Staining Quick Guides Table 1: Mouse Cytokines: Intracellular Staining Quick
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
al. 2006b ). MATERIALS Reagents Anti-CD24 monoclonal antibody (mAb) (J11d clone) (for Protocol 3) Use either culture supernatant that contains anti-CD24 mAb or purified anti-CD24 mAb (see Step 55). BDPharmLyse (red blood cell lysing
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