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文献和实验A Novel Method for the Production of Fully Modified K-Ras 4B
post-translationally modified proteins in bacterial expression systems is often problematic. Here, we describe a novel recombinant approach to prepare human K-Ras 4B, a protein that is post-translationally farnesylated
Assaying Cdc25 Phosphatase Activity
To determine the activity of all human Cdc25 phosphatases, two different methods are described. For assaying phosphatase activities of recombinant Cdc25 proteins produced in Escherichia coli or insect cells, a fluorimetric assay using
Genetic and Pharmacologic Models for Type 1 Diabetes
. Diabetes 42: 44‐ 55. Craighead, J.E. 1975. Animal model of human disease. Mice infected with the M variant of Encephalomyocarditis virus. Am. J. Pathol. 76: 537‐ 540
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