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文献和实验Isolation of Human Monoclonal Antibodies Using Guided Selection with Mouse Monoclonal Antibodies
Repertoires of antibody (Ab) V genes derived from nonimmunized human donors (1 ) or made synthetically (2 3 ) have been cloned for display on filamentous bacteriophage as either scFvs or Fabs fused to the minor phage coat protein (pIII
【翻译】Development trends for monoclonal antibody cancer therapeutics
7 and patients frequently developed antibodies to the mouse-derived proteins, which limited their utility8, 9. In addition, only certain murine mAb isotypes have been shown to effectively bind to and activate elements of the human immune
Method for Purification of Human Hematopoietic Stem Cells by Flow Cytometry
Human hematopoietic stem cells (HSCs) and progenitors can be isolated by enriching for a rare cell population with a combination of monoclonal antibodies (MAbs). Such an isolation scheme involves multi-step procedures including
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