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文献和实验Culturing Mouse Embryonic Fibroblasts
to human fibroblasts (Parrinello S., et al. Nature Cell Biol, 2003 ). A. Isolating Primary MEFs: B. Passaging primary MEFS: PROCEDURE Setup mouse breeder pairs in the afternoon. Ideally one male
Basic Protocol 1: Direct Detection of Blood Vasculature in Tissue with Prefixation of Vasculature Materials Mouse
, 0.1mM ßME. plus 3 U/mL Epo, 50 ng/mL Tpo, 100 ng/mL SCF, 100 ng/mL IL-6, ? ng/mL IL-3. Antibodies for Lineage Depletion: (Pharmingen) Antibodies for Sorting: (Pharmingen monoclonal rat anti-mouse)
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