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文献和实验Immunohistochemisty-Fluorescence Protocol
antibody (either biotin-donkey anti-goat IgG Fab2 diluted 1:700 or biotin-goat anti-mouse IgG1 or IgG2A or IgG2B diluted 1:500) in Wash Buffer-Saponin. Wash the cells twice in Wash Buffer-Saponin. Incubate the centrifuged cell pellet
Immunohistochemistry: Fluorescence Protocol 2.0
by centrifugation (400 x g for 5 minutes). Incubate the cells for 30 minutes at room temperature with 50 µL of a biotinylated secondary antibody (either biotin-donkey anti-goat IgG Fab2 diluted 1:700 or biotin-goat anti-mouse IgG1 or IgG2A or IgG2B diluted
, QC, Canada; Catalog No. 11 277 073 910). Recommended for single FISH. 6. Biotin RNA labeling mix (Roche Applied Science; Catalog No. 11 685 597 910). 7. RNAguard (Amersham Biosciences, Piscataway, NJ, USA; Catalog No. 27-0816-01).
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