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文献和实验) and unrelated proteins by formaldehyde groups. The great majority of monoclonal and polyclonal antibodies used for immunohistochemical (IHC) staining of formalin-fixed, paraffin-embedded (FFPE) tissues necessitate unmasking antigens for antigen retrieval
8.5. After dehydration with dimethylformamide, the specimens are embedded in LR-White resin. For antigen retrieval, ultrathin sections are heated in 20 mM Tris–HCl buffer (pH 9.0) for 1 h at 95�C. After immunogold labeling, the sections are treated with a mixture
Mouse p27 PCR Using Gitschier Buffer
: 1 M Tris pH 8.8 (do not use pH meter, store at room temp.) 1.23 g Tris HCl 5.13 g Tris base q.s. 50 mL with H2 O KG-1 (10x) (Store frozen) Amt [final] 8.3 mL 1M (NH
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