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999
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100 ml
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文献和实验实验试剂 Tween-20 (Sigma Cat. # P-7949); BSA (Sigma Cat. # A-7030); ABTS Liquid Substrate Solution (Sigma Cat. # A3219); Dulbecco’s PBS [10x] (Gibco BRL Cat. #14200-075). RECOMMENDED SOLUTIONS All solutions
免疫组化技术-Immunohistochemical Protocols
2) or a biotin conjugated (Table2) secondary antibody is used, both at a dilution of 1:500 in BSA diluent for 1 hr, Wash in PBS 3x5 min Incubate in avidin-biotin-peroxidase complex (ABC reagent
product code P-1379). Make up to 0.6 litres with distilled water. This is a 10x concentrate which should be diluted with distilled water as required (eg 150ml diluted with 1350ml of distilled water). * In most applications, 10mM phosphate, 0.15 M NaCl
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