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文献和实验Isotype Determination of Monoclonal Antibodies by Immunodiffusion
antibodies are allowed to diffuse toward anti-mouse isotype specific antisera in an agarose gel. In a positive reaction, a precipitin line will develop in the agarose between two wells. A monoclonal antibody should show only one heavy chain and one light
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
on mouse tissue are of IgG1 isotype. Detailed explanation of this method can be found in: Cattoretti G, Qing Fei. Application of the Antigen Retrieval Technique in experimental Pathology: from human to mouse
Rapid Isotyping of Mouse Monoclonal Antibodies
Determination of class and subclass of monoclonal antibodies (MAbs) is important because several immunochemical and functional properties of antibodies depend on the isotype. Knowledge of the light-chain isotype (κ for 95% or λ
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