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文献和实验The production of monoclonal antibody by hybridoma
We outline a simple and contemporary protocol for the development of monoclonal antibodies using hybridoma fusion in immune mice (1). While the basic style of this fusion is similar to others (2,3), this protocol
, 0.1mM ßME. plus 3 U/mL Epo, 50 ng/mL Tpo, 100 ng/mL SCF, 100 ng/mL IL-6, ? ng/mL IL-3. Antibodies for Lineage Depletion: (Pharmingen) Antibodies for Sorting: (Pharmingen monoclonal rat anti-mouse)
Phenotype-specific immunodetection of cyclins using 488/630 nm dual laser flow cytometry
-cyclin D1 (Pharmingen cat. no. 14561A, clone G12-4326) or cyclin D2 /D3 (cat. no. 14711A, clone G107-22). Other cyclin antibodies may be appropriate as well. Cy5-conjugated anti-mouse IgG (Jackson ImmunoResearch and Caltag) FITC-conjugated
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