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文献和实验Studies of the Ubiquitin Proteasome System
Assays of E1 and E2 Activity Basic Protocol 1: Thiolester Formation Between Rabbit E1 and Ubiquitin Basic Protocol 2: Thiolester Formation Between E2 and Ubiquitin Binding of Ubiquitin‐Proteasome Proteins Basic Protocol 3: Binding of E2s to E3s Alternate
to measure proteasome activity, evaluate its state of assembly, and assess the accumulation and aggregation of ubiquitinated proteins in two types of neuronal cultures: human neuroblastoma cells and rat primary cortical cultures. These protocols can be used
Chromatin Assembly Using Drosophila Systems
Alternate Protocol 1: Expression and Purification of the Recombinant Drosophila NAP‐1 (NTA Superflow Resin) Basic Protocol 6: Chromatin Assembly with Purified Recombinant Drosophila Factors Alternate Protocol
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