Recombinant Bacillus Circulans

Site-Specific Protein Labeling by Intein-Mediated Protein Ligation

Intein-mediated protein ligation (IPL) employs an intein to create a protein possessing a C-terminal thioester that can be ligated to a protein or peptide with an amino-terminal cysteine via a native peptide bond. Here we present a procedure

Protein and Vitamin Production in Bacillus megaterium

The Gram-positive bacterium Bacillus megaterium is frequently employed for the industrial production of exoenzymes including amylases, proteinases, and penicillin acylases, for instance. Here, we describe the utilization of the excellent

Antibody Production by the Gram-Positive Bacterium Bacillus megaterium

The increasing demand for recombinant antibodies as detection reagents in research, diagnostics, and therapy requires appropriate production systems. In contrast to antibody therapies, small recombinant antibody fragments like Fab and scFv