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文献和实验Mutational Analysis of the Human Protein C Gene
(2 ,3 ). The gene is approximately 11 kb long and is composed of 9 exons and 8 introns. In common with factors VII, IX and X the exons encode specific structural domains of the protein C molecule. Exon 1 encodes the 5′ untranslated region, exon 2 encodes the signal
Detection of Phosphatidylinositol-4-Phosphate 5-Kinase Activity Using Thin-Layer Chromatography
by the PI 3-kinase, generating phosphatidylinositol 3,4,5-trisphosphate (PI 3,4,5-P3 ), a second messenger of generally unknown function; however, some isoforms of protein kinase C may be targets (2 ) as well as proteins that contain pleckstrin homology (PH
Introduction of human 14-3-3 proteins
inhibitor of protein kinase C,but the N-terminal domain does not inhibit PKC activity.Site-specific mutagenesis of several regions in the tau isoform of 14-3-3,including the mutation of a putative pseudosubstrate site to a potential substrate sequence
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