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文献和实验Liposomes and Phospholipid Binding Proteins in Glycoprotein Biosynthesis
of the protein as a cotranslational event; and (3) the modification of the protein-linked oligosaccharide by removal and addition of sugar residues (processing) as the glycoprotein proceeds through the ER and Golgi complex (see ref. 1 for a comprehensive review
Zinc-Finger Nucleases-Based Genome Engineering to Generate Isogenic Human Cell Lines
Customized zinc-finger nucleases (ZFNs) have developed into a promising technology to precisely alter mammalian genomes for biomedical research, biotechnology, or human gene therapy. In the context of synthetic biology, the targeted
Purification of the human TATA-Binding Protein, TBP
The TATA-binding protein TBP plays a central role in eukaryotic transcription initiation (1 , 2 ). TBP binds to the promoter consensus sequence TATAAA, located approx 30 nucleotides upstream of the transcriptional start site. Once bound, TBP
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