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文献和实验Immunofluorescent Staining of Mouse and Rat Leukocytes
receptors), a three-step protocol may amplify the staining: use purified primary antibody (steps 2-4 of above procedure), biotinylated anti-Ig for the 2nd step (steps 6-7, above), and fluorochrome-conjugated avidin or streptavidin as the 3rd step (repeat
Antigen Design Sera Purification Tech Sheet
control measures (mass spec and/or analytical HPLC). An indirect synthesis of the MAP can eliminate analysis problems. In the indirect method, the peptide is first synthesized, purified then analyzed using mass spec and analytical HPLC. The peptide antigen
T-cell dependent antigen specific in situ staining (Pe)
if you have them). Let them dry approx 1 hour in a dry atmosphere at RT. Antibodies (anti-Pe) in the sections degrade more rapidly than tissue antigens. Proceed with staining within 24 hours or freeze the slides. Antigen-specific staining: (Pe staining of Pe
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