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文献和实验Testing Rabbit Bleeds By Elisa
same amount of NaBH4 after 15''. Do a quick microfuge spin if there are too many bubbles. B. ELISA with Peptide conjugated to BSA Coat 10 ug/ml antigen (peptide conjugated to BSA) diluted in TBS (50 ul/well) ON at 4 deg C. Remove antigen
Immunofluorescence: Rabbit Anti-Murine RELMα
. Following fixation, the tissue was embedded in paraffin and cut into 5 μM sections. 1. Deparaffinize and rehydrate the tissue section. 2. Perform heat-induced antigen retrieval by boiling the tissue section in 10mM pH 6.0 citrate buffer
Testing Rabbit Bleeds By Elisa
Testing Rabbit Bleeds By Elisa Claire Walczak January 1996 You can test whether or not you have gotten an immune response to the peptide and how strong that immune response is by doing ELISAs against peptide conjugated to BSA
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