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- Cedarlane
- CLHIV135
- 2025年07月11日
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- 库存：
999
- 规格：
100 µg
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文献和实验Nonradioactive Labeling and lmmunodetection of Recombinant Proteins
, or Western blot can be carried out using enzyme-labeled primary or secondary antibodybased systems or it can be performed using biotmylated primary or secondary antibodies with streptavidin or avidin-enzyme conlugates. Biotin is often the hapten of choice
Dual- and Triple-Color Fluorospot
in the analysis, e.g., IL-10, rat mAb 9D7 is used for coating and tag (different from FITC and biotin)-labeled rat mAb 12G8 is used for detection. All mAbs from Mabtech, Nacka Strand, Sweden. 4. Secondary reagents for amplification: Mouse anti-FITC mAb
or polyhistidine-tagged recombinantly expressed bait proteins are often detected with a primary or enzyme-labeled antibody specific to the tag. Antibodies to both these popular fusion tags are commercially available. When recombinant techniques cannot be used
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