Recombinant HIV-1 gp41

【求助】HIV-gp41原核表达

秦奋 为什么HIV-gp41与原核PET28a质粒构建后，行诱导表达，SDS-PAGE无结果，而WB却有结果，这种情况怎么回事？（构建后测序同源性达95%，而且已酶切认证构建成功） 谢谢 zhujoker “SDS-PAGE无结果，而WB却有结果”是什么意思，你表述清楚一点，要不人家会有疑惑的。其实这主要看你是否诱导成功。个人认为还有就是你在原核里面表达，作为包涵体存在形式，你是否将蛋白提出来了呢？

Competitive Antigen Panning for Selection of HIV-1 Neutralizing Human Monoclonal Antibodies Specific for gp41

HIV envelope glycoprotein (Env) is composed of two non-covalently associated subunits: gp120 and gp41. Panning of phage-displayed antibody libraries against gp140 (covalently linked gp120 and the extracellular portion of gp41) has resulted

Evaluation of Compounds Against Recombinant HIV Reverse Transcriptase

Reverse transcriptase (RT) has attracted particular attention as a target enzyme for AIDS chemotherapy, because the enzyme catalyzes a crucial step in the HIV replicative cycle. Effective inhibition of this enzyme prevents the formation