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文献和实验Transformation of E. coli by Electroporation
Materials: SOB medium E. coli host strain such as DH5α WB tRNA 5 M ammonium acetate
Transformation of E. coli Reference: H. Inoue, H. Nojima & H. Okayama, Gene 96, 23-28 (1990) 1. Culture E. coli in 250 ml of SOB medium in a 2-liter flask at 18 C with vigorous shaking until an A 260
The magic E. coli transformation protocol
The magic E. coli transformation protocol Take the ultra-competent cells out of freezer. Thaw on ice. Put your selection plates (LB +?) on the bottom shelf of an incubator to pre-warm them (about 37 to 45o C) Add half
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