Rat Albumin (purified antigen)

Immunofluorescent Staining of Mouse and Rat Leukocytes

receptors), a three-step protocol may amplify the staining: use purified primary antibody (steps 2-4 of above procedure), biotinylated anti-Ig for the 2nd step (steps 6-7, above), and fluorochrome-conjugated avidin or streptavidin as the 3rd step (repeat

Dual- and Triple-Color Fluorospot

for the other cytokine (Fig. 1). As a second step in the detection, streptavidin conjugated to a red fluorophore (Cy3) and anti-FITC antibodies labeled with a green fluorophore, respectively, are used (10, 11). The resulting spots are subsequently analyzed using

Large-scale immunofluorescence

antibodies   Texas Red-conjugated anti-rabbit (Jackson Labs, 1:200 dilution) FITC-conjugated donkey anti-rat (Jackson ImmunoResearch Laboratories, 1:100 dilution) Cy3-conjugated goat anti-mouse (Jackson Labs, 1:300 dilution)