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文献和实验Immune Response Biomarker Profiling
实验试剂 ProtoArray® Human Protein Microarray v5.0 Blocking Buffer and Washing Buffer 10 × Synthetic Block [ 详细信息： Invitrogen ] Alexa Fluor® 647 Goat Anti-Human IgG [ 详细信息： Invitrogen ]
by reaction with alkaline phosphatase-conjugated goat anti-mouse secondary antibody. Choose the actin pool broadly, however, consider also checking the column fractions by dot blot with mAB 3F2 to identify capping protein. Pool the actin fractions
Immunofluorescent staining of Sea Urchin embryos
1. Transfer fixed embryos to microfuge tubes. Allow to settle for 10 minutes. Gently remove most of the liquid. 2. Add 100 ul antibody to one tube and 100 ul 1% normal goat serum to the other as a control. Let sit for 45 minutes
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