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文献和实验) fraction. Peroxidase conjugated Protein A Cappel #55901. Guinea Pig anti-actin Animal #2 whole serum, Botstein lab, prepared by Jon Mulholland. Sample Buffer: 1ml 0.5M TRIS pH6.8 + 4.4ml ddH2O + 2.0ml 10% SDS + 2.0ml glycerol + 1.0ml 1M
) fraction. Peroxidase conjugated Protein A Cappel #55901. Guinea Pig anti-actin Animal #2 whole serum, Botstein lab, prepared by Jon Mulholland. Sample Buffer: 1ml 0.5M TRIS pH6.8 + 4.4ml ddH2O + 2.0ml 10% SDS + 2.0ml glycerol + 1.0ml 1M
them individually with different fluorochrome conjugated secondary antibodies? (FITC-conjugated anti-Mouse IgG 1 will label both!) The biotin-avidin system may provide an answer, if one antibody can be biotinylated: label with the non-biotinylated mouse antibody
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