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文献和实验Immunodetection of cyclin D1 and D2/D3 using flow cytometry
in PBS with 100 U/ml RNase. Analyze on any 488 nm argon- 630 nm HeNe or diode dual laser flow cytometer for PI and Cy5 fluorescence. Both PI and Cy5 should be analyzed in linear mode. Recipes Anti-cyclin D1 (Pharmingen cat. no. 14561A, clone G12
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
Staining mouse tissue with anti-mouse Ig (H&L chain) secondary reagents inevitabily causes staining of interstitial immunoglobulins, B cells, plasma cells and macrophages with Ig bound to Fc
Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometr（图）
the need for fluorescence compensation. This assay can be used with any instrument employing dual laser excitation, including the B-D Vantage, FACSCalibur and Coulter Elite. Materials Anti-cyclin D1 (Pharmingen cat. no. 14561A, clone G12-4326) or cyclin
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