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文献和实验GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
.V. and Neel B.G. 1993. Solubilization and purification of enzymatically active glutathione-S- transferase (GEX) fusion proteins. Anal. Biochem . 210 : 179-187. Sambrook J. and Russell D.W. 2001. Molecular Cloning: A Laboratory Manual
in cellular glutathione (GSSG) level is correlated with the formation of S-glutathionated protein, a mixed-disulfide exchange reaction has been proposed to be responsible for this protein modification (2 –5 ,9 –10 ). However, glutathione (GSH) modified
Purification of Baculovirus-Expressed Human DNA Topoisomerase I
for htopo I constructs fused to either the T7 gene 10 translation initiation signal (6 ) or to glutathione-S-transferase (GST). Furthermore, expression in protease-deficient strains of E. coli does not appreciably reduce the proteolytic breakdown of htopo
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