Catalase Assay Kit (without Hy

CAT是一种普遍存在的抗氧化酶，参与H2O2的解毒，H2O2是正常有氧代谢和致病性ROS产生的有毒产物。它催化两个H2O2分子转化为分子氧和两个水分子（催化活性）。CAT还表现出过氧化活性，其中低分子量醇可以作为电子供体。虽然脂肪族醇是CAT的特定底物，但其他具有过氧化活性的酶不利用这些底物。Cayman的过氧化氢酶检测试剂盒利用CAT的过氧化功能来测定血浆、血清、红细胞裂解物、组织匀浆和细胞裂解物中的酶活性。该方法基于酶与甲醇在最佳浓度的H2O2存在下的反应。产生的甲醛用Purpald比色法测量，Purpald是一种色原体，它与醛专门形成双环杂环，氧化后会从无色变为紫色。CAT is a ubiquitous antioxidant enzyme involved in the detoxification of H2O2, a toxic product of both normal aerobic metabolism and pathogenic ROS production. It catalyzes the conversion of two molecules of H2O2 to molecular oxygen and two molecules of water (catalytic activity). CAT also demonstrates peroxidatic activity, in which low molecular weight alcohols can serve as electron donors. While aliphatic alcohols serve as specific substrates for CAT, other enzymes with peroxidatic activity do not utilize these substrates. Cayman's Catalase Assay Kit utilizes the peroxidatic function of CAT for determination of enzyme activity in plasma, serum, erythrocyte lysates, tissue homogenates, and cell lysates. The method is based on the reaction of the enzyme with methanol in the presence of an optimal concentration of H2O2. The formaldehyde produced is measured colorimetrically with Purpald, a chromogen that specifically forms a bicyclic heterocycle with aldehydes, which upon oxidation changes from colorless to a purple color.分子式分子量