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文献和实验Use of Tissue-Plasminogen Activator as a Reporter Gene
Throughout the past six or seven years, our ability to study and understand the process of eukaryotic gene expression has been greatly enhanced by the use of reporter genes. In essence, a reporter gene encodes a protein product
as the Northern blot (2 ). Both are, then, often key elements in establishing the identity of nucleic acids of interest. Northern blot analysis was used by us as a tool in order to answer the question whether or not the nuclear transcripitional apparatus of human
The determination of the protein content of urokinase-type plasminogen activator (uPA) and its inhibitor, PAI-1, in breast cancer tissue extracts is used clinically to identify patients at risk to experience disease recurrence (metastasis
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