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文献和实验Visualization of the Expression of Green Fluorescent Protein (GFP)-Linked Proteins
of the jellyfish, Aequoria victoria (1 , 2 ). The original expression plasmid for this jellyfish protein, green fluorescent protein (GFP) yielded a low level of fluorescence. However, through mutagenesis, enhanced GFPs have been produced that are about 35 times
of a promoter-probe plasmid that utilizes a variant of green fluorescent protein (GFP) as its reporter. The technique has been used successfully to identify novel Salmonella typhimurium genes that are selectively expressed following exposure to acid
Quantitative immunoprecipitation of GFP-fusion proteins using the GFP-Trap
Introduction Green fluorescent proteins (GFP) and derivates thereof are widely used to study protein localization and dynamics in living cells (Heim and Tsien, 1996; Tsien, 1998). The validation and interpretation of these data
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