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- 2025年07月14日
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文献和实验purifying and identifying a broad spectrum of SUMO-conjugated proteins and a focused approach for purifying a selected SUMO target and mapping its SUMO attachment site(s). Although both methods were initially developed for use in S. cerevisiae
Detecting Low‐Affinity Extracellular Protein Interactions Using Protein Microarrays
. Biol. Chem. 272:30889‐30898. Kerr, J.S. and Wright, G.J. 2012. Avidity‐based extracellular interaction screening (AVEXIS) for the scalable detection of low‐affinity
Purification of DNA‐Binding Proteins Using Biotin/Streptavidin Affinity Systems
of DNA‐binding proteins using the biotin/streptavidin affinity technique. The protocol involves the following steps: (1) a biotinylated, labeled DNA fragment is prepared containing a binding site for the protein to be purified; (2) the biotin‐cellulose
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