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- 2025年07月10日
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
, during time series analyses or when screening transfor-mants for expression of a transformed gene. Under such circumstances, existing tech-niques are overly time consuming and yield more RNA than is necessary. The availability of a rapid RNA mini-prep
Mini DNA extraction from wheat leaves for PCR and Southern B
till homogeneous. 7. Centrifuge 10 minutes at room temperature. (If you centrifuge at 4 ℃, SDS may re-precipitate.) 8. Draw off 400 μl supernatant into a new 2 ml tube containing 1 ml of 100 % ethanol. 9. Allow your sample to sit undisturbed for at least 15
Bacterial Protein Extraction (mini-scale) Using B-Per
Bacterial Protein Extraction (mini-scale) Using B-Per B-Per (Pierce, Cat number: 78248) Extraction of 1.5ml bact.culture OD600nm 1.5-3.5 A useful procedure for initial screening of expression
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