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文献和实验Purification of Human Multiprotein Complexes using OneSTrEP Technology (PROT41)
buffer 2 mM D-biotin (Sigma, B4501) (Use 10 mM Biotin for batch purification) Strep-Tactin Superflow® columns IBA Tagnology (Germany), Cat. No. 2-1207-550
, and 2) fractionation over BlueSepharose, which binds Wnts with high selectivity. The purificationconsists of four chromatography steps: Blue Sepharose, immobilized metal affinity chromatography (IMAC), gel filtration,and heparin cation exchange
protein depends upon the species that the antibody was raised in. Protein A binds well to rabbit, cat, human, pig and guinea pig IgG as well as mouse IgG2a and IgG2b. Protein G binds strongly to IgG from cow, goat, sheep, cow, horse, rabbit and guinea pig
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