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文献和实验Secondary antibody: Goat anti-mouse alkaline phosphatase conjugated - Biorad #170-6520. Substrate: ECF chemifluorescent substrate - Amersham #RPN5785. Mix substrate with accompanying buffer as per manufacturer’s
Alternate Protocol 1: RNA Preparation from Pure RPE Cells Basic Protocol 4: Protein Preparation from Eye Tissues Basic Protocol 5: Fixation, Inclusion, and Cryosection of the Mouse Eye
Antibody Production: Activity-Dependent Neuroprotective Protein (ADNP) as an Example
in incomplete Freund’s adjuvant, the respective antisera were collected and assayed by enzyme-linked immunosorbent assay (ELISA) and purified by affinity chromatography on peptides conjugated to SulfoLink Coupling Gel (Pierce). Mouse brain proteins were prepared
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