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- CLAS15-2944
- 2025年07月15日
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文献和实验Isolate It All：siRNA • miRNA • Total RNA • Native Protein
denaturant does not significantly affect RNA quality. The advantage is that this lysate contains native protein that can be used directly in a host of downstream applications [2, 5], such as two-dimensional gel electrophoresis (Figure 5B).
7.5, 150 mM NaCl, and1% (v/v) Triton X-100. 7. Suitable SDS-PAGE and transfer setup as in Section 2.4.1 . 8. Protein Sample Loading Dye (4×) as in Section 2.4.1 . 9. Nitrocellulose or PVDF membrane. 10. Mouse anti-β-catenin antibody (available
Staining Procedure for Flow Cytometric Detection of Human Cyclins
unconjugated isotype controls and mAbs, add 20 m l of FITC-conjugated goat anti-mouse Ig (Cat. No. 12064D) at optimal working dilution. Incubate 30 min at RT in the dark. Add 2 ml Wash buffer, centrifuge 5 min at 200 x g Aspirate supernatant. 10
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