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文献和实验Heterologous SERCA1a Ca2+ -ATPase (sarco-endoplasmic reticulum Ca2+ -adenosine triphosphatase isoform 1a) from rabbit was expressed in yeast Saccharomyces cerevisiae as a fusion protein, with a biotin acceptor domain (BAD) linked to the SERCA
Synchronizing Protein Transport in the Secretory Pathway
as a reporter was constructed as described in . The Interleukin‐2 signal peptide was inserted between Asc I and Eco RI and the GPI anchor between Fse I and Pac I sites. The retention occurs in the lumen using Str‐KDEL as a hook. HeLa cells were transiently
v468.chapter 1 经典 Wnt/b-Catenin 信号通路 (英文完整版)
complexes on target cellslocated up to 20–30 cell diameters distant (8 , 9) . Wnts start out as precursors containing an N-terminalhydrophobic signal peptide that directs the immature proteinto the endoplasmic reticulum (ER). In the ER, the signal
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