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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
dilutions for rabbit polyclonal antibodies range between 1:50 and 1:500. For double-labeling experiments, use primary antibodies raised in two different species. Antibodies, secondary Secondary antibodies are diluted in PBS containing 2% normal
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
, and mix Enzyme Conjugate: phosphate‐buffered saline containing avidin linked to horseradish peroxidase, protein, enzyme stabilizers and preservative (18 ml per bottle; from Competitive ELISA
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
biomarkers. Curr. Protoc. Protein Sci. 64:27.2.1?27.2.26. © 2011 by John Wiley & Sons, Inc. Keywords: protein microarray; antibodies; serum profiling
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