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文献和实验IAA Analysis in Transgenic Plants
-conjugates remains a vital part of the analysis if we are to understand auxin action. Several methods are available utilizing immunoassays, GC-MS, high-performance liquid chromatography (HPLC), and fluorescence. One reliable method for IAA analysis
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
【资源】[共享]Adhesion Protein Protocols——（很有用的）
Materials 2.1— Construction of Adhesion Molecule—Ig Chimeras 2.2— Electroporation of Cells 2.3— Harvest and Culture of Transfectant Clones 2.4— Selection (ELISA) and Subcloning 2.5— Purification of Chimeric Proteins 3— Methods 3.1—
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