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文献和实验Studies of the Ubiquitin Proteasome System
Basic Protocol 9: Substrate Ubiquitylation by Multisubunit E3s Materials VBC‐Cul2 E3 protein complex expressed and purified using baculoviral expression system ( protocol 45 ) Mouse E1
LINEAGE ANALYSIS USING RETROVIRAL VECTORS
, but are not limited with respect to the ubiquitous phospholipid receptor for the VSV G protein [16]. We did not see this effect of increased efficiency of infection using murine vectors and murine embryos, but it is possible that different mouse strains vary
. PBTB solution: 1x PBS, 0.1% Tween-20, 1% milk powder. 4. Detection of DIG-labeled probe: a. Biotinylated anti-DIG antibody followed by Streptavidin-HRP, recommended to obtain strongest signal: Biotin-conjugated mouse
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