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文献和实验Antigen Design Sera Purification Tech Sheet
, it is important to avoid self-recognition of the immunogen by the host animal. As an example, when raising antibodies against a human protein, it is more suitable to use a rabbit or mouse host than a monkey. For highly conserved mammalian proteins, raising
免疫组化技术-Immunohistochemical Protocols
Incubation in affinity purified indocarbocyanine (Cy3, viewed in red field, 1:1000 in BSA diluent) or fluorescein isothiocyanate (FITC, viewed in green field, 1:100 in BSA diluent) fluorochrome conjugated secondary antibodies (F[ab']2 fragments
Studies of the Ubiquitin Proteasome System
(Promega) 20 to 40 U/µl ribonuclease inhibitor (e.g., RNasin; Promega) 10 mM NTP mix (10 mM each ATP, CTP, GTP, UTP; Promega) 0.5 + 2µg/µl plasmid vector containing cDNA for E2 of interest under the control
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