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文献和实验Measurement of Phosphoinositide 3-Kinase Activity
class IA PI 3-kinase is a heterodimer consisting of the 85 kDa regulatory subunit (responsible for protein-protein interactions either via protein tyrosine phosphate-binding SH2 domains or SH3 domains and/or proline rich regions) and a catalytic 110 kDa
Two-hybrid analysis of genetic regulatory networks
, E. A. (1994). Ste5 tethers multiple protein kinases in the MAP kinase cascade required for mating in S. cerevisiae. Cell 78 , 499-512. Durfee, T., Becherer, K., Chen, P.-L., Yeh, S.-H., Yang, Y., Kilburn, A. E., Lee, W.-H., and Elledge, S. J
Identifying Protein Interactions by Hydroxyl‐Radical Protein Footprinting
: A method for studying protein‐DNA interactions. Methods Enzymol. 130:132‐181. Casaz, P. and Buck, M. 1999. Region I modifies DNA‐binding domain conformation of sigma 54
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