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文献和实验Measurement of Mitochondrial DNA Copy Number
dependent on a number of nuclear proteins. Defects in these nuclear genes can lead to mitochondrial DNA (mtDNA) depletion (Spinazzola et al. Biosci Rep 27:39–51, 2007). The mitochondrial DNA (mtDNA) depletion syndromes (MDDSs) are autosomal recessive
Gene Editing in One‐Cell Embryos by Zinc‐Finger and TAL Nucleases
immunoglobulin gene disruption and targeted replacement in rabbit using zinc finger nucleases. PLoS One 6:e21045. Gallagher, S. R. 2011. Quantitation of DNA and RNA with absorption
T-cell dependent antigen specific in situ staining (Pe)
HRP-conjugated secondary Ab choice: 10- add the HRP-conjugated Goat anti rabbit (Vector 50 to 100 µl, dilution 1:100) and incubate for 30’. Swine anti-rabbit (Dako) can be used at 1:300 dilution. If you use this step for tyramide
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