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文献和实验The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
reagent; BD Biosciences 555899) (for Protocol 4) Buffer for cell staining and cell sorting (for Protocol 4; see Step 72) Complement, reconstituted from rabbit (Cedarlane CL3331) (for Protocol 3) ESC medium (for Protocols 1, 2) Freezing
【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
39611β-HSD 1B variantI, active548.0Human serum albuminS, 13.4mg/g biomass536InsulinS, synthetic signal or α-MF, 1.5 g/L, active[233], 532Insulin-like growth factor-1 (IGF-1)S, 600 mg/L, α-MF[234Interferon α-1S, α-MF, 35 mg/L, active520Interferon gamma
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
) supplemented with 10% (v/v) foetal bovine serum (FBS; Gibco; Invitrogen, Carlsbad, CA), penicillin/streptomycin(Gibco) and 10 μM retinoic acid to induce neuronaldifferentiation (see Note 1 ). 2. Insulin-like growth factor (IGF)-1 (Invitrogen) was dissolvedin
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