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文献和实验Antigen Design Sera Purification Tech Sheet
and may have some degree of secondary structure. Coupling Strategy A factor that is often over-looked when designing a synthetic peptide is the method of coupling the peptide to the carrier protein. For example, N-terminal sequences should be coupled through the C
Antigen Design Sera Purification Tech Sheet
A factor that is often over-looked when designing a synthetic peptide is the method of coupling the peptide to the carrier protein. For example, N-terminal sequences should be coupled through the C-terminal amino acid and vice versa for C
Studies of the Ubiquitin Proteasome System
antibody for the protein of interest and isotype‐matched control antibody, e.g., irrelevant or preimmune serum, purified Tris‐buffered saline/Tween 20 (TBST; see recipe ), 4°C Cells growing in culture
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