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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
solution ( 36.5%; Sigma-Aldrich 33220) Glycine (2 M; Fisher BP381) Glycogen (Roche) H2 O, nuclease-free Immune complex wash buffers (high-salt and low-salt) Prepare the high-salt and low-salt versions of this buffer
Detection of Protein‐Protein Interactions by Coprecipitation
interactions between known proteins and as a means of identifying components of a complex. This unit describes basic approaches to immunoprecipitating tagged proteins from whole?cell extracts. The extract is prepared under nondenaturing conditions, the protein
Application of Amide Proton Exchange Mass Spectrometry for the Study of Protein‐Protein Interactions
Basic Protocol 2: Determination of the Solvent Accessibility of a Protein‐Protein Interface Via the Off‐Exchange Method H 2 O
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