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文献和实验Immunohistochemistry: Mouse Anti-Human MCP-1
. 4. Incubate the tissue section overnight at 4?C with Mouse Anti-Human MCP-1 at 10.0 μg/mL. Wash the slide twice for three minutes each. 5. Incubate the tissue section with the Envision HRP polymer kit (Dako) according to the manufacturer’s
The Chimeric Human/Mouse Model of Angiogenesis
3 ). This process is similar to, but not identical with vasculogenesis, which is associated with the development of blood vessels from precursor cells termed angioblasts (4 ,5 ). Under normal physiological conditions the complex cellular events controlling
Culture of Human Embryonic Stem Cells on Human and Mouse Feeder Cells
This chapter describes the methods we use to maintain and expand undifferentiated human embryonic stem (hES) cells on human and mouse feeder cells. All of the available hES cells have been derived and propagated on primary mouse embryonic
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