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文献和实验to a sterile 10 ml Falcon tube, and pump up and down to release keratinocytes from the epidermis. Pass the suspension through a sterile, 70μm nylon filter (Becton Dickinson) into a fresh 50 ml Falcon tube to remove cornified sheets. Rinse the initial tube
【转帖】Top 100 proteomics publications
Top 100 proteomics publications ----From Ion Channel Media Group：ttp://www.ionchannelmedia.com/websites.php 1. Gavin et al. (2006) Proteome survey reveals modularity of the yeast cell machinery. Nature 2. Bonetta et al. (2006) Protein
BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method
by centrifugation at 5K rpm for 15 minutes in a 250 ml bottle in the RC5-B using the GSA rotor, cell pellets should be frozen and stored at -70 degrees C. 2、Prior to use, the cells from 200 ml growth were thawed and resuspended in 8 ml of just 10 mM of EDTA, pH
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