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文献和实验Immunohistochemistry using Anti-Ganglioside Antibodies
')2 or FITC-conjugated goat anti-mouse IgG F (ab')2 diluted at 1:1000 each with 1% BSA in PBS for 1 hr. at room temperature. 7. Wash with 1% BSA in PBS 5 times. 8. Apply drops of fluorescence mounting medium on section and mount micro cover glass
Immunohistochemistry using Anti-Ganglioside Antibodies
')2 or FITC-conjugated goat anti-mouse IgG F (ab')2 diluted at 1:1000 each with 1% BSA in PBS for 1 hr. at room temperature. 7. Wash with 1% BSA in PBS 5 times. 8. Apply drops of fluorescence mounting medium on section and mount micro cover glass
In situ immunodetection of 5-methylcytosine (5-mC) on squashed cells using anti-methylcytosine
dry 4) Block slides in PBST + 1% BSA for 20 min at RT 5) Incubate slides in monoclonal anti-5-mC (1:1000 dilution in PBS) for 1h at 37oC in a humid chamber 6) Wash in PBST 2x5min 7) Incubate slides in Biotin anti-mouse IgG (1:500 dilution
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