Anti-Human IgG Fd Ab, Biotin,

Isotype-specific immunostaining with mouse primary antibodies on mouse tissue

on mouse tissue are of IgG1 isotype. Detailed explanation of this method can be found in: Cattoretti G, Qing Fei. Application of the Antigen Retrieval Technique in experimental Pathology: from human to mouse

Purification of mAb (IgG)

buffer (pH9.0) ~ 200 ml. Filter protein mixture Add protein mixture or culture supernatant containing Ab (adjust pH to 7.8 with Binding buffer ; red color) to the Protein A column. Mouse antibodies of the IgG1 subclass

Immunohistochemistry using Anti-Ganglioside Antibodies

')2 or FITC-conjugated goat anti-mouse IgG F (ab')2 diluted at 1:1000 each with 1% BSA in PBS for 1 hr. at room temperature. 7. Wash with 1% BSA in PBS 5 times. 8. Apply drops of fluorescence mounting medium on section and mount micro cover glass