Anti-Human IgG2 Fc, Purified,

Exploiting Fc Chimaeric Proteins for the Identification of Ligands Specific for the Mannose Receptor

. In this chapter we describe our experience in the use of proteins containing selected regions of the mannose receptor fused to the Fc region of human IgG1 or murine IgG2b. Using these reagents we have discovered new and unexpected ligands for the mannose receptor

Capture of the Human IgG1 Antibodies by Protein A for the Kinetic Study of h-IgG/FcR Interaction Using SPR-Based Biosensor Technology

either from purified antibodies or from crude cell media supernatants are captured by the protein A and are used as the ligand. Soluble forms of FcγRs are injected at different concentrations and are used as the analyte. This setup allows the definition of the kinetic

Protein Therapeutics: Mouse, Humanized, and Human Antibodies

component of the vertebrate immune system. Each plasma cell secretes a single clone of antibodies that bind a unique epitope of an antigen. Cooperations exist between antibodies and other immune effectors such as macrophages and complement to facilitate