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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 规格:
0.1 mg
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文献和实验, and the resultant mixture was stirred for an appropriate time at room temperature. After completion of the reaction as indicated by thin-layer chromatography (TLC), the reaction mixture was washed with NH4Cl solution. The product was extracted with ethyl acetate
RNase-free DNaseI (10 U/µl), 5 µl 0.1 M Tris-Cl pH 8.3, 5 µl 0.5 M KCl, 5 µl 15 mM MgCl 2 phenol extract once precipitate with 5 µl NaAcetate and 200 µl 100 % Ethanol incubate at least for 30 min at -80 °C spin, wash
Bacic Immunoprecipitation(免疫沉淀实验)
and isotype of the primary antibody. The methods described are comparable and the choice of method depends on the specific antigen-antibody system. 主要 试剂 Agarose conjugate: Protein A immobilized on Sepharose® CL-4B [Details
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